Peptide reconstitution calculator
Select your peptide, bacteriostatic water volume, and research dose to calculate concentration, syringe draw volume, and total applications — step by step.
Peptide & bacteriostatic water
Choose your peptide vial — concentration is calculated automatically from water volume.
Research dose & syringe
Desired amount per application and insulin syringe size.
U-100 insulin syringe · 100 units = 1.0 ml
How this is calculated
Concentration = Vial (mcg) ÷ Water (ml) = 10,000 ÷ 2 = 5,000 mcg/ml
Draw (ml) = Dose (mcg) ÷ Concentration = 250 ÷ 5,000 = 0.050 ml
Total doses = Vial (mcg) ÷ Dose = 40 applications
Step-by-step mixing instructions
For your selected · 10 mg vial · 2 ml water
- 1
Prepare your workspace
Clean your work surface with alcohol. Gather your peptide, bacteriostatic water, a 0.3 ml insulin syringe, and alcohol swabs.
- 2
Equalize vial pressure
Draw air into the syringe (about the same volume as water you will add) and inject it into the peptide vial. This reduces vacuum and helps prevent foaming.
- 3
Draw bacteriostatic water
Using a sterile syringe, draw 2 ml of bacteriostatic water. Tap gently and expel air bubbles from the syringe.
- 4
Reconstitute the peptide
Inject water slowly down the inside wall of the vial — not directly onto the lyophilized powder — to protect peptide integrity.
- 5
Dissolve completely
Gently swirl the vial in circular motions until fully dissolved. Do not shake. The solution should be clear before use.
- 6
Measure your research dose
Draw 5.0 units (0.050 ml) on your 0.3 ml syringe. This delivers 250 mcg per application.
- 7
Store properly
Refrigerate reconstituted peptide at 2–8°C, upright and protected from light. Use within 28 days for optimal stability in most research protocols.
Storage & stability
- Lyophilized: Store frozen or refrigerated per product COA. Stable for extended periods when sealed.
- Reconstituted: Refrigerate at 2–8°C. Use within 28 days for most peptides.
- Avoid repeated freeze-thaw cycles and direct light exposure.
Pro tips
- Always inject water down the vial wall — never directly onto the powder.
- Gently swirl to dissolve; shaking can damage peptide structure.
- Wipe the vial stopper with an alcohol swab before each access.
- Use a new sterile syringe for each draw to maintain sterility.
Unit reference
| mcg | mg |
|---|---|
| 100 | 0.1 |
| 250 | 0.25 |
| 500 | 0.5 |
| 1,000 | 1 |
| 2,500 | 2.5 |
For research and educational purposes only. Peptide Lab products are not intended for human or animal consumption. Always verify calculations independently and follow your institution's laboratory protocols.